Free Peptide Reconstitution Calculator for Researchers

Accurate reconstitution is the foundation of reliable peptide research. This free calculator eliminates manual math errors by instantly computing your working concentration, mcg-per-syringe-unit conversion, and exact syringe draw volume for any lyophilized peptide and solvent combination. Select from 29 pre-loaded compounds from our research catalog or enter custom values for any peptide in your workflow.

Enter your peptide vial size (mg) and the volume of bacteriostatic water you plan to add. The calculator returns your concentration in mg/mL and mcg/mL, the micrograms per syringe unit, and — if you enter a target dose — the exact number of insulin syringe units to draw, complete with a visual syringe fill indicator. For a complete walkthrough of the reconstitution process itself, see our step-by-step reconstitution guide.

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How to Use This Calculator

Using the calculator takes three steps. First, select your peptide from the dropdown — this auto-fills the standard vial size and recommended solvent volume. Second, adjust the values if needed for your specific vial or protocol. The calculator instantly updates your concentration in mg/mL, mcg/mL, and mcg per syringe unit. Third, if you need to measure a specific microgram dose, enter it in the optional dose field and the calculator tells you exactly how many syringe units to draw, with a visual fill indicator.

Understanding the Results

Concentration (mg/mL)

The most fundamental result — milligrams of peptide per milliliter of solution. Calculated as total peptide (mg) divided by total solvent volume (mL). Example: 5 mg of BPC-157 in 2 mL of bacteriostatic water = 2.5 mg/mL.

Concentration (mcg/mL)

The same value in micrograms (multiply mg/mL by 1,000). More practical for dosing since most peptide protocols work in microgram quantities. A concentration of 2.5 mg/mL equals 2,500 mcg/mL.

Micrograms per Syringe Unit

A standard U-100 insulin syringe divides 1 mL into 100 units. This tells you how many micrograms each unit mark represents. If your concentration is 2,500 mcg/mL, each syringe unit contains 25 mcg — so drawing 10 units delivers exactly 250 mcg.

Syringe Units for a Specific Dose

Enter a desired dose in micrograms and the calculator determines the exact syringe units to draw. Formula: units = (desired mcg ÷ concentration in mcg/mL) × 100.

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Quick-Reference Reconstitution Volumes

Standard volumes used in common research protocols. Select any compound in the calculator dropdown to auto-fill these, or use them as a starting reference:

• BPC-157 (5 mg) → 2 mL BAC water → 2.5 mg/mL
• TB-500 (5 mg) → 2 mL BAC water → 2.5 mg/mL
• Ipamorelin (5 mg) → 2.5 mL BAC water → 2 mg/mL
• CJC-1295 No DAC (2 mg) → 2 mL BAC water → 1 mg/mL
• Semaglutide (5 mg) → 2.5 mL BAC water → 2 mg/mL
• Tirzepatide (5 mg) → 2.5 mL BAC water → 2 mg/mL
• Retatrutide (5 mg) → 2.5 mL BAC water → 2 mg/mL
• Melanotan II (10 mg) → 2 mL BAC water → 5 mg/mL
• AOD9604 (5 mg) → 2.5 mL BAC water → 2 mg/mL
• Sermorelin (2 mg) → 2 mL BAC water → 1 mg/mL

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Choosing the Right Solvent

The standard reconstitution solvent for most research peptides is bacteriostatic water — sterile water preserved with 0.9% benzyl alcohol, which inhibits bacterial growth and allows multiple withdrawals over up to 28 days.

Some peptides, particularly larger or more hydrophobic compounds like Semaglutide and Liraglutide, may require acetic acid solution (0.6–1%) for complete dissolution at higher concentrations. If your peptide doesn’t dissolve in bacteriostatic water within 5 minutes of gentle rolling, switch to acetic acid. For full solvent guidance, see our reconstitution guide.

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Frequently Asked Questions

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